abstract：:Fluoxetine (Prozac) is an antidepressant that is thought to act by blocking presynaptic reuptake of the neurotransmitter serotonin. Despite widespread clinical use of fluoxetine, direct evidence for this mechanism has been difficult to obtain in vivo. We have determined that fluoxetine has an additional neuromuscular ...

journal_title：Molecular cell

authors： Choy RK,Thomas JH

更新日期：1999-08-01 00:00:00

abstract：:Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI spli...

journal_title：Molecular cell

authors： Cramer P,Cáceres JF,Cazalla D,Kadener S,Muro AF,Baralle FE,Kornblihtt AR

更新日期：1999-08-01 00:00:00

abstract：:SNAP proteins play an essential role in membrane trafficking in eukaryotic cells. They activate and recycle SNARE proteins by serving as adaptors between SNAREs and the cytosolic chaperone NSF. We have determined the crystal structure of Sec17, the yeast homolog of alpha-SNAP, to 2.9 A resolution. Sec17 is composed of...

journal_title：Molecular cell

authors： Rice LM,Brunger AT

更新日期：1999-07-01 00:00:00

abstract：:The recruitment of the TATA box-binding protein (TBP) to promoters in vivo is often rate limiting in gene expression. We present evidence that TBP negatively autoregulates its accessibility to promoter DNA in yeast through dimerization. The crystal structure of TBP dimers was used to design point mutations in the dime...

journal_title：Molecular cell

authors： Jackson-Fisher AJ,Chitikila C,Mitra M,Pugh BF

更新日期：1999-06-01 00:00:00

abstract：:The crystal structure of the autoinhibited form of Hck has been determined at 2.0 A resolution, in complex with a specific pyrazolo pyrimidine-type inhibitor, PP1. The activation segment, a key regulatory component of the catalytic domain, is unphosphorylated and is visualized in its entirety. Tyr-416, the site of act...

journal_title：Molecular cell

authors： Schindler T,Sicheri F,Pico A,Gazit A,Levitzki A,Kuriyan J

更新日期：1999-05-01 00:00:00

abstract：:Initiation sites for DNA synthesis in the chromosomal autonomously replicating sequence (ARS)1 of Saccharomyces cerevisiae were detected at the nucleotide level. The transition from discontinuous to continuous synthesis defines the origin of bidirectional replication (OBR), which mapped adjacent to the origin recognit...

journal_title：Molecular cell

authors： Bielinsky AK,Gerbi SA

更新日期：1999-04-01 00:00:00

abstract：:We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and Sec7p. Specific residues within the Sec7 domain are important for BFA inhibition of AR...

journal_title：Molecular cell

authors： Peyroche A,Antonny B,Robineau S,Acker J,Cherfils J,Jackson CL

更新日期：1999-03-01 00:00:00

abstract：:We have used EM visualization of active genes on plasmid vectors in Xenopus oocyte nuclei to investigate the relationship between poly(A) signals and RNA polymerase II transcription termination. Although a functional poly(A) signal is required for efficient termination, cotranscriptional RNA cleavage at the poly(A) si...

journal_title：Molecular cell

authors： Osheim YN,Proudfoot NJ,Beyer AL

更新日期：1999-03-01 00:00:00

abstract：:Various experiments have demonstrated a collaborative action of Myc and Ras, both in normal cell growth control as well as during oncogenesis. We now show that Ras enhances the accumulation of Myc activity by stabilizing the Myc protein. Whereas Myc has a very short half-life when produced in the absence of mitogenic ...

journal_title：Molecular cell

authors： Sears R,Leone G,DeGregori J,Nevins JR

更新日期：1999-02-01 00:00:00

abstract：:The pre-mRNA 5' splice site is recognized by the ACAGA box of U6 spliceosomal RNA prior to catalysis of splicing. We previously identified a mutant U4 spliceosomal RNA, U4-cs1, that masks the ACAGA box in the U4/U6 complex, thus conferring a cold-sensitive splicing phenotype in vivo. Here, we show that U4-cs1 blocks i...

journal_title：Molecular cell

authors： Kuhn AN,Li Z,Brow DA

更新日期：1999-01-01 00:00:00

abstract：:B and T cell receptor gene assembly by V(D)J recombination is tightly regulated during lymphoid development. The mechanisms involved in this regulation are poorly understood. Here we show that nucleosomal DNA is refractory to V(D)J cleavage. However, the presence of HMG1, a chromatin-associated nonhistone DNA-binding ...

journal_title：Molecular cell

authors： Kwon J,Imbalzano AN,Matthews A,Oettinger MA

更新日期：1998-12-01 00:00:00

abstract：:In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...

journal_title：Molecular cell

authors： Cleghon V,Feldmann P,Ghiglione C,Copeland TD,Perrimon N,Hughes DA,Morrison DK

更新日期：1998-12-01 00:00:00

abstract：:We obtained a recessive insertion mutation in the gene encoding yeast TBP-associated factor yTAFII61/68 that impairs Gcn4p-independent and Gcn4p-activated HIS3 transcription. This mutation also reduces transcription of seven other class II genes, thus indicating a broad role for this yTAFII in RNA polymerase II transc...

journal_title：Molecular cell

authors： Natarajan K,Jackson BM,Rhee E,Hinnebusch AG

更新日期：1998-11-01 00:00:00

abstract：:The S. cerevisiae SCFCdc4p ubiquitin-protein ligase complex promotes cell cycle transitions through degradation of cell cycle regulators. To investigate SCFCdc4p regulation in vivo, we examined the stability of individual SCFCdc4p components. Whereas Cdc53p and Skp1p were stable, Cdc4p, the F box-containing component ...

journal_title：Molecular cell

authors： Zhou P,Howley PM

更新日期：1998-11-01 00:00:00

abstract：:A basis for understanding specificity of molecular recognition between phosphorelay proteins has been deduced from the 2.6 A structure of the Spo0B phosphotransferase of the phosphorelay regulating sporulation initiation. Spo0B consists of two domains: an N-terminal alpha-helical hairpin domain and a C-terminal alpha/...

journal_title：Molecular cell

authors： Varughese KI,Madhusudan,Zhou XZ,Whiteley JM,Hoch JA

更新日期：1998-10-01 00:00:00

abstract：:BRCA1 and BRCA2 account for most cases of familial, early onset breast and/or ovarian cancer and encode products that each interact with hRAD51. Results presented here show that BRCA1 and BRCA2 coexist in a biochemical complex and colocalize in subnuclear foci in somatic cells and on the axial elements of developing s...

journal_title：Molecular cell

authors： Chen J,Silver DP,Walpita D,Cantor SB,Gazdar AF,Tomlinson G,Couch FJ,Weber BL,Ashley T,Livingston DM,Scully R

更新日期：1998-09-01 00:00:00

abstract：:Activation of NF-kappaB is achieved by ubiquitination and proteasome-mediated degradation of IkappaBalpha. We have detected modified IkappaBalpha, conjugated to the small ubiquitin-like protein SUMO-1, which is resistant to signal-induced degradation. In the presence of an E1 SUMO-1-activating enzyme, Ubch9 conjugated...

journal_title：Molecular cell

authors： Desterro JM,Rodriguez MS,Hay RT

更新日期：1998-08-01 00:00:00

abstract：:Two cyclin-dependent kinases have been identified in yeast and mammalian RNA polymerase II transcription initiation complexes. We find that the two yeast kinases are indistinguishable in their ability to phosphorylate the RNA polymerase II CTD, and yet in living cells one kinase is a positive regulator and the other a...

journal_title：Molecular cell

authors： Hengartner CJ,Myer VE,Liao SM,Wilson CJ,Koh SS,Young RA

更新日期：1998-07-01 00:00:00

abstract：:Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor e...

journal_title：Molecular cell

authors： Wells SE,Hillner PE,Vale RD,Sachs AB

更新日期：1998-07-01 00:00:00

abstract：:MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is increased when Mre11 is in a complex with Rad50. Mre11 also exhibits endonuclease activ...

journal_title：Molecular cell

authors： Paull TT,Gellert M

更新日期：1998-06-01 00:00:00

abstract：:The hairpin ribozyme is a self-cleaving motif found in the negatives strand of the satellite RNA of some plant viruses. In its natural context, the ribozyme comprises four helices, two of which contain conserved formally unpaired loops, that are adjacent arms of a four-way RNA junction. We show that the arms that woul...

journal_title：Molecular cell

authors： Murchie AI,Thomson JB,Walter F,Lilley DM

更新日期：1998-05-01 00:00:00

abstract：:The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...

journal_title：Molecular cell

authors： Zhong H,Voll RE,Ghosh S

更新日期：1998-04-01 00:00:00

abstract：:Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS d...

journal_title：Molecular cell

authors： Graveley BR,Maniatis T

更新日期：1998-04-01 00:00:00

abstract：:HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...

journal_title：Molecular cell

authors： Margottin F,Bour SP,Durand H,Selig L,Benichou S,Richard V,Thomas D,Strebel K,Benarous R

更新日期：1998-03-01 00:00:00

abstract：:In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cyt...

journal_title：Molecular cell

authors： Kutay U,Lipowsky G,Izaurralde E,Bischoff FR,Schwarzmaier P,Hartmann E,Görlich D

更新日期：1998-02-01 00:00:00

abstract：:The Cdc28p cyclin-dependent kinase is thought to both catalyze the onset of DNA replication and prevent rereplication by blocking the reassembly of initiation complexes at replication origins. Budding yeast with mutations in the CDC16 gene represent an exception to this model, because they rereplicate DNA despite bein...

journal_title：Molecular cell

authors： Heichman KA,Roberts JM

更新日期：1998-02-01 00:00:00

abstract：:Two yeast enzymes that catalyze aminoacylation of tRNAs, MetRS and GluRS, form a complex with the protein Arc1p. We show here that association of Arc1p with MetRS and GluRS is required in vivo for effective recruitment of the corresponding cognate tRNAs within this complex. Arc1p is linked to MetRS and GluRS through i...

journal_title：Molecular cell

authors： Simos G,Sauer A,Fasiolo F,Hurt EC

更新日期：1998-01-01 00:00:00

abstract：:Mammalian cells constantly monitor and respond to a myriad of extracellular signals, often by using cell surface receptors. Two important classes of cell surface receptors include the receptor tyrosine kinases, which recognize peptide growth factors such as insulin, and the integrins, which most often mediate binding ...

journal_title：Molecular cell

authors： Shrivastava A,Radziejewski C,Campbell E,Kovac L,McGlynn M,Ryan TE,Davis S,Goldfarb MP,Glass DJ,Lemke G,Yancopoulos GD

更新日期：1997-12-01 00:00:00

abstract：:We have used gene competition to distinguish between possible mechanisms of transcriptional activation of the genes of the human beta-globin locus. The insertion of a second beta-globin gene at different points in the locus shows that the more proximal beta gene competes more effectively for activation by the locus co...

journal_title：Molecular cell

authors： Dillon N,Trimborn T,Strouboulis J,Fraser P,Grosveld F

更新日期：1997-12-01 00:00:00